MICROSCOPY:


AURORA

AIRY BEAM LIGHT SHEET MICROSCOPY IMAGING SYSTEM

  • Wide field of view - image larger specimens faster than other systems
  • Isotropic axial resolution - image samples from any angle
  • Low photo bleaching - image specimens for longer
  • Modular design - customisable system to suit your research needs
  • Intuitive operation – minimal training required
  • Programmable - Develop your own functions/code
  • Flexible specimen preparation – use live, fixed and cleared specimens (incubation and perfusion facilitated)

Aurora is an innovative, compact, flexible and affordable single and/or multiphoton light sheet fluorescence imaging system for rapid, large 3D volumetric imaging, high resolution, multicolour, time-lapse imaging and live cell imaging.

Airy beam Light Sheet Microscopy (ABLSM) is an increasingly popular imaging modality for visualising whole samples, such as embryos and organoids, and is used for the longtime observation of embryonal development in different model organisms in developmental biology, cell biology, neuroscience and regenerative medicine.

Aurora is already being used out in the field by many leading organisations with outstanding results.

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CORE FEATURES

The entry level Aurora system is a compact bench-top light system with a footprint of 60 x 60 x 30 cm and features:

  • Sub-cellular 1-micron isotropic resolution
  • Peak Irradiance of 80% less than a Gaussian light sheet, whilst maintaining a similar axial resolution
  • 20x larger X-Y field of view than a Gaussian light sheet
  • 500x faster than confocal microscopy
  • 10x increase in signal-to-noise after Airy deconvolution

The entry-level system can be easily adapted or upgraded for a multitude of applications. Additional modules include:

  • Wide range of continuous wave laser lines available
  • Multiphoton light sheet imaging configuration
  • Fully or partially automated stage
  • Environmental control

aurora custom development programme

  • Joining the Aurora customisation programme places the configuration and specifications of an instrument directly in your hands, so that your microsope meets the exacting needs of your research requirements. The benefits of the programme include:

  • PERSONALISED PROGRAMME

    Tailor the programme to suit your budget or timescales

  • DEVELOP A CUSTOM SYSTEM

    Choose from a range of modules and create a system to suit your research requirements

  • HANDS-ON-TRAINING

    Practical assistance to help you optimise sample imaging protocols

  • PRIORITY TECHNICAL SUPPORT

    Help from our dedicated application specialists and engineers

  • GRANT APPLICATION SUPPORT

    Access available funds with the support of our grant writing team

  • CO-AUTHORED PAPERS

    Develop papers in conjunction with us and/or other programme members

  • PREFERENTIAL PURCHASE TERMS

    Programme members receive preferential purchase terms

GET IN TOUCH WITH OUR SPECIALISTS

IMAGE CREDITS

*1 3D volumetric projection of
 2 adult C. elegans specimens expressing fluorescent protein in their body wall muscles. Original dataset was 600x600x600 micrometers.

Strains courtesy of Dr Serena Ding, MRC London Institute of Medical Sciences. Samples prepared by Dr Muna Elmi, University College London. Images captured by Dr Mike Shaw, National Physical Laboratory.

*2 3D volumetric projection of a Meristem (leaf shoot) of an Arabodopis plant expressing GFP-CHDR5 (Magenta) overlaid with the plant’s native autofluorescence (Cyan). Original dataset was 600x600x1,000 micrometres.

Image courtesy of Tom Timmers, Max Planck Institute for Plant Breeding Research.

*3 3D volumetric projection of a stitched dataset of 3 Z-stacks. It shows a living 2-day old zebrafish from head to tail mid-body, labelled with GFP-sox17 (Cyan) and RFP-prox1(Yellow). Original dataset was 1,700x600x400 micrometres.

Image courtesy of Professor Lene Broeng Oddershede, Dr Younes Farangebarooji and Dr Elke Ober, Niels Bohr Institute Copenhagen.

*4 3D volumetric projection of a live 2 Day Old Zebrafish Tail, labelled with GFP-sox17-cyan, RFP-prox1-magenta. Original dataset was 600x600x400 micrometres.

Image courtesy of Professor Lene Broeng Oddershede, Dr Younes Farangebarooji and Dr Elke Ober, Niels Bohr Institute Copenhagen.

*5 Progression through a Z-stack of a 1 week-old Zebrafish Larvae with Transgenic Expression of GCaMP in the brain. Original dataset was 600x600x400 micrometres.

Image courtesy of Thomas Shallcross and Thomas Sainsbury, MRC Centre - Developmental Neurobiology, King's College London.

*6 3D volumetric projection of an optically cleared mouse brain expressing thy1-GFP Actin. Original dataset was 600x600x200 micrometres.

Image courtesy of Dr Anthony Vernon and Robert Chesters, Department of Basic and Clinical Neuroscience, Maurice Wohl Clinical Neuroscience; MRC Centre for Neurodevelopment Disorders, King's College London.

*7 3D volumetric projection of mouse hindbrain expressing GFP- CX3CR1 (Green) overlaid with the native autofluorescence (Magenta). Original dataset was a multi-photon excited volume of 600x600x200 micrometres.

Image courtesy of Dr Anthony Vernon and Robert Chesters, Department of Basic and Clinical Neuroscience, Maurice Wohl Clinical Neuroscience; MRC Centre for Neurodevelopment Disorders, King's College London.

*8 Maximum intensity projection of a living 2-day old zebrafish tail, labelled with GFP-sox17 (Cyan) and RFP-prox1(Magenta). Original dataset was 600x600x400 micrometres.

Image courtesy of Professor Lene Broeng Oddershede, Dr Younes Farangebarooji and Dr Elke Ober, Niels Bohr Institute Copenhagen.

*9 Maximum intensity projection of 2-week old mouse intestine organoids labelled with 4 colours. DAPI staining the nuclei (Blue), AF647-Phalloidin staining Actin (Red), AF555-WGA staining the Paneth cells (Yellow), and GFP-LGR5 expressing in the stem cells (Green). Original dataset was 600x600x200 micrometres.

Image courtesy of Dr Sandra Scharaw and Dr Sylvie Le Guyader, Karolinska Institute, Department of Biosciences and Nutrition.

*10 Maximum intensity projection of an optically cleared mouse brain labelled with Alexa 488-Neurofilament (Cyan) and Alexa 568-Parvalbumin (Red). Original dataset was 800x800x1,000 micrometres.

Image courtesy of Adam Tyson, MRC Centre Developmental Neurobiology, King's College London.

*11 Maximum intensity projection of an optically cleared mouse brain expressing thy1-GFP Actin using a coloured height map. Original dataset was 600x600x640 micrometres.

Image courtesy of Dr Anthony Vernon and Robert Chesters, Department of Basic and Clinical Neuroscience, Maurice Wohl Clinical Neuroscience; MRC Centre for Neurodevelopment Disorders, King's College London.

*12 Maximum intensity projection of an optically cleared mouse brain expressing thy1-GFP Actin using a coloured height map. Original dataset was 600x600x1,000 micrometres.

Image courtesy of Dr Anthony Vernon and Robert Chesters, Department of Basic and Clinical Neuroscience, Maurice Wohl Clinical Neuroscience; MRC Centre for Neurodevelopment Disorders, King's College London.

LASER ENQUIRIES

+44 (0)141 945 0500

Head Office

 

+1-720-242-8190

USA, East Coast

 

+1-650-798-5040

USA, West Coast

MICROSCOPE ENQUIRIES

+44 (0)1483 502 003

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