Multiphoton Imaging Abounds at SfN Neuroscience 2011

The annual meeting of the Society for Neuroscience (SfN), often referred to simply as "Neuro", is arguably the premier academic meeting of its kind in the world for all things related to the science of the brain.  It provides a forum for researchers to present and discuss their latest work.  In addition it hosts an enormous trade show exhibition with a wide range of products from laboratory consumables to complex systems for imaging the brain.

This year's show was held in Washington D.C. in mid November and attracted a grand total of 32,000 attendees.

Light plays a key role in the study of the brain. Microscopy techniques can image deep into living samples or break the classical optical resolution limit (so called super-resolution) to give information on the finest details of the structures of the brain.  Light and neuroscience mix in the exciting field of optogenetics (Nature's method of the year 2010). This was evident in the trade show with several microscope vendors displaying their products. 

The laser plays a major part in many of these microscope products as the "engine" that, for example, generates the fluorescence that in turn generates the image.  One specific technique called multiphoton excitation (MPE) microscopy enables images of slices of the brain to be made without the need to physically slice the brain tissue.  This technique requires lasers that produce a train of short pulses of light - a technique known as mode locking. These short pulses only interact with the tissue in small volumes at the focal region of the microscope and so limit damage to the living sample. This allows multiple optical slices to made and and then combined to create a 3-dimentional picture of the structures. 

The number of microscope vendors offering such systems has certainly increased in recent years and more than ten vendors were offering such systems this year, double the number of only a few years ago.

The Sprite laser from M Squared produces the short intense pulses of light used for this technique.

Next years show is in New Orleans.

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